ISIS Elixir for Life Reduced Fat Colostrum

By reducing the fat without destroying any elements in the colostrum we are able to concentrate the
immunoglobulins and growth factors, along with all proteins and related elements.   This also makes the product
even more suitable for lactose free diets.  Proteins are increased from approximately 50% to 76%.  The PRPs are
reduced, but not removed from the colostrum,  The higher dosage taken by those who are symptomatic, or by
athletes, still provide sufficient and relevant amounts of PRPs.  There is no known toxicity level and larger doses,
as much as 60 grams, have proven to be beneficial.


Description and Suggested Applications

EFL_APS-40 is a pasteurized reduced fat, high IgG colostrum powder produced from raw colostrum collected only
from milkings within 16 hours after birth. EFL_ APS-40 is processed both at low pressures and temperatures and is
spray dried using indirect steam to maintain maximum bio-activity.  The raw colostrum used to produce EFL_APS-
40 is from antibiotic free sources.  

Suggested applications include: immune system enhancement, nutritional supplement, digestive health
improvement, protein supplement and alternative treatment for general health.

Major Active Component Analysis

                                                   Specification                  Typical                      Method              

Protein % (Nx6.38) db                            68.0 min                       76.0                        Kheldal
Total Immunoglobulins %                        40.0 min                       47.0                        HPLC (dry basis)
Immunoglobulins (Type G1 & G2) %        32.0 min                       40.0                        HPLC (dry basis)
Immunoglobulins (Type A) %                   2.50 min                       3.70                        HPLC (dry basis)
Immunoglobulins (Type M) %                   1.75 min                       2.50                        HPLC (dry basis)
Immunoglobulins (Type D) %                   0.30 min                       0.40                        HPLC (dry basis)
Immunoglobulins (Type E) %                   0.20 min                       0.30                        HPLC (dry basis)
Lactoferrin %                                        1.0 min                       1.75                        HPLC (dry basis)
Transferrin (mg/g)                                     4.0 min                       6.20                        HPLC (dry basis)
Lactoperoxidase-thiocyanate %                 0.8 min                       1.00                        HPLC (dry basis)
Proline-Rich Polypeptides (PRPs) %          0.5 min                       0.80                        HPLC (dry basis)
Insulin Growth Factor (Type 1) µg/g          2.3 min                       3.00                        ELISA (dry basis)
Insulin Growth Factor (Type 2) ng/g         150 min                        200                        ELISA (dry basis)
Derived Platelet Growth Factor ng/g          5.0 min                       6.00                        HPLC (dry basis)
Epidermal Growth Factor µg/g                   1.0 min                       2.00                        ELISA (dry basis)
Fibroblast Platelet Growth Factor ng/g       6.0 min                       8.00                        ELISA (dry basis)
Transforming Growth Factor Alpha ng/g    25.0 min                    32.0                         ELISA (dry basis)
Transforming Growth Factor Beta mg/g      0.30 min                       0.40                     ELISA (dry basis)
Nerve Growth Factor ng/g                         15.0 min                       20.0                        ELISA (dry basis)
Leptin ng/g                                               60.0 min                       70.0                        ELISA (dry basis)

Vitamin Analysis*

Vitamin B6 µg/g                                        5.0 min                         10.0                Mass spec. (wet basis)
Vitamin B12 µg/g                                    0.05 min                         0.10                Mass spec. (wet basis)
Vitamin E µg/g                                         0.05 min                         0.10                Mass spec. (wet basis)
Vitamin A µg/g                                         5.00 min                         7.00                Mass spec. (wet basis)
Vitamin C µg/g                                         0.10 min                         0.15                Mass spec. (wet basis)
Thiamin (Vitamin B1)µg/g                        20.0 min                            50                Mass spec. (wet basis)
Folic Acid µg/g                                        0.50 min                         0.75                Mass spec. (wet basis)
Pantothenic Acid µg/g                             0.50 min                         1.00                Mass spec. (wet basis)
Riboflavin (Vitamin B2) µg/g                   10.0 min                         15.0                Mass spec. (wet basis)
Beta-carotene µg/g                                    50 min                            70                Mass spec. (wet basis)
Glycoconjugates µg/g                             0.50 min                         1.20                Mass spec. (wet basis)
Glycogen µg/g                                        20.0 min                         34.0                Mass spec. (wet basis)
Retinoic Acid µg/g                                    2.0 min                           4.00               Mass spec. (wet basis)

                                
Analytical and Microbiological Analysis*
              Specification              Typical                     Method         
Moisture %                                               5.0 max                   4.50                        CEM
Protein % (Nx6.38) db                             68.0 min                   77.0                         Kheldal
Ash %                                                       9.0 max                     6.00                       Mass Spec.
Fat %                                                        4.0 max                     2.00                       Mojonnier
Lactose %                                               12.0 max.                  11.0                        by difference
Sediment                                                      B                            A                          Pad
pH                                                           6.5 < 7.5                     7.0                       10% sol., 20C
Bulk density                                          0.25 < 0.35                   0.27                     grams / cc
Standard Plate Count (col/g)                 30,000 max                5,000                      Standard**
Yeast and Mold (col/g)                              10 max                     <10                       Standard**
Coliform (col/g)                                         10 max.                     <10                      3M Petrifilm
E.Coli (col/g)                                            10 max.                      <1                        3M Petrifilm
Salmonella sp.                                       Neg. / 25g                    Neg.                     ELISA
Listeria sp.                                            Neg. / 25g                     Neg.                     ELISA

Essential Amino Acid Analysis* (weight/weight basis)(GC/MS)        Non Essential Amino Acid Analysis*(w/w) (GC/MS)
Isoleucine                                           1.46%                                  Arginine                                2.30%
Leucine                                              2.37%                                 Cystine                                1.12%
Histidine                                             1.46%                                 Glutanic Acid                        9.13%
Methionine                                         4.08%                                 Alanine                                2.50%
Lysine                                                4.18%                                 Tyrosine                                4.96%
Threonine                                          4.03%                                 Glycine                                1.77%
Phenylalanine                                    2.42%                                 Proline                                5.12%
Valine                                                2.16%                                 Aspartic Acid                        5.57%
Tryptophan                                        1.17%                                 Serine                                4.77%
               
Other Minor Components not Quantified
Beta 2- microglobulin, Enzymes, Haemopexin, Haptoglobulin, Orotic Acid, Peroxidase, Xanthine Oxidase Enzyme,
Gonadotropin-Releasing Hormone (GnRH), Prolactin, Insulin, Sulfur, Glycoproteins: - (Including Protease and
Trypsin Inhibitors), Lactalbumin, Multimeric a-Lactalbumin, Cytokines, Lysozymes, Gamma Globulin, B
Lactoglobulin, Complement 3 & 4 (C3 & C4), Kappa Casein, Alpha 2-AP glycoprotein, Alpha 1- antitrypsin, Alpha 2-
macroglobulin, Orosomucoids, Prealbumin, Albumin, Oligosaccharides, Non Specific Inhibitors (NSI’s), Secretory
IgA (SigA), IgA Specific Helper

Mineral Analysis* and Physical Properties
mg/100gm                Specification     Typical             Heavy Metals                   Properties

Sodium                     50 < 150           80                Lead        <1ppm              Solubility:   Good
Calcium                    50 < 150           80                Mercury    <1ppm              Flavor:      Clean & Bland
Potassium                50 < 150            80               Arsenic     <1ppm              Odor:         Clean
Magnesium               70 < 100            80                                                        Appearance: Free Flowin  
Chromium                  30 < 60             40                                                            & non-caking
Zinc                          30 < 60             40                           
Chloride                        < 60              50                                                     

Packaging, Shipping and Storage

Colostrum proteins are hygroscopic and can absorb odors.  Temperatures below 75 degrees F, relative humidity
below 65% and an odor free environment will extend storage life.  Stocks should be used in rotation and preferably
within three years or by date posted.  It is best not to freeze this product.   

* Where not specified results are reported on “ as is” basis except.    ** Standard Methods for Examination of Dairy
Products 16th Edition



                                                                     Manufactured for ISIS Nutraceuticalsby APS™, LLC